RNA sequencing and immunofluorescence of the myotendinous junction of mature horses and humans

The myotendinous junction (MTJ) is a specialized interface for transmitting high forces between the muscle and tendon yet MTJ common site of strain injury with recurrence rate. aim this study was to identify previously unknown components in mature animals humans. Samples were obtained from superficial digital flexor (SDF) muscle-tendon 20 horses, tissue separated through sequential cryosectioning approach into muscle, (muscle enriched myofiber tips attached tendon), fractions. RT-PCR performed genes known be expressed three fractions t-distributed stochastic neighbor embedding (t-SNE) plots used select MTJ, samples five horses RNA sequencing. expression identified sequencing studied by immunofluorescence on human hamstring tissue. main finding that panel 61 at MTJ. Of these, 48 novel 13 had been reported associated earlier studies. [COL22A1 (collagen XXII), NCAM (neural cell adhesion molecule), POSTN (periostin), NES (nestin), OSTN (musclin/osteocrin)] undescribed [MNS1 (meiosis-specific nuclear structural protein 1), LCT (lactase)] confirmed level sections In conclusion, tips, we representing diverse biological processes, which may important maintenance